Description
It is a rapid phosphatase test for the detection of alcaline phosphatase in milk, whey, cream and butter. The test allows the testing of sufficient pasteurisation treatment of milk and related products, and the detection of raw milk in pasteurized milk. Sensitivity of the test is as follows:
For measurement from 5 seconds up to 300 seconds: 3.5 mU/L ALP
One kit is enough for 100 tests. For the test procedure there is no need of thermal preparation of the sample. The test works at temperatures 2-42 °C. The kit with the reagents must be stored at the refrigerator at a temperature 2-8 °C.
Comparison with other tests
| Test | Incubation | Time result | Temperature | Equipment | Sensivity level | Samples |
| LACTOPAST Biomedix | no | 5 sec | 2-42 °C | no | 3.5 mU/L | Pasteurised milk, HTST, Chocolate milk, creams, cheese, whey, butter |
| CHARM F-AP | no | 45-90 sec | Room temp. | Luminometer, centrifuge | 20 mU/L | HTST, chocolate milk, creams |
| CHARM PASLite | 3 min. | 45-90 sec | Room temp. | Luminometer, centrifuge, spectrophotometer | 20 mU/L | HTST, chocolate milk, creams |
| SENSOBIZ APT | no | 5-10 min. | Room temp | no | 120 mU/L | Pasteurised milk, HTST |
| MACHEREY-NAGEL Phosphatesmo MI | 60 min. | Read results after incubation | 36 °C | incubator | 1750 mU/L | Pasteurised milk |
| BIOO SCIENTIFIC MaxSignal | 30 min. | Read results after incubation | 37 °C | incubator | 700 mU/L | Pasteurised milk, cheese |
| Heyl Lactognost III | 60 min. | 10 min. | 37 °C | Incubator, centrifuge, water bath | 350 mU/L | Pasteurised milk, whey, cream, butter |
| HARDY Alkaline Phosphatase | 30-60 min. | Read results after incubation | 37 °C | Water bath, incubator | 700 mU/L | Pasteurised milk, butter, cream, cheese |
| HYGIENA ZymoSnap | 10 min. in 20-25 °C + 5 min. in 37 °C | Read results after 15 min. | Room temp. | Luminometer, waterbath | 222 mU/L | Pasteurised milk, creams, HTST |
