Description
Total Aflatoxin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Total Aflatoxin (B1, B2, G1, G2) in cereals, meat/fish, feed, milk ,oils,peanuts and pistachios .
The unique features of the kit are:
– High recovery (80-105%), cost-effective extraction methods.
– High sensitivity (0.05 ng/g or ppb).
– High reproducibility.
– Quick (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The second antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Content of the kit
1 x 96 wells microplate (42measurement in duplicate, from which 12 are used for the standards) with Aflatoxin coating.
Aflatoxin standard with different concentrations (0.05 to 0.8 ng/ml)
Antibody 1 and Conjugate Antibody 2, wasching solution, substrate for the enzymtic reaction, stop buffer
Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.
