Description
This system to measure oxygen (DO) and pH in microplates can be used inside an incubator and is ideally suited for high-throughput screenings, cell cultivations or a multitude of parallel respiration measurements. These special well plates, the PhoxyPlates, have optical sensors integrated in each well that are read out non-invasively through the transparent plate bottom with the readout unit. The innovative image-based read out makes PhoxyCube compatible with variable plate geometries. Currently well plates in 24-well plate format with both oxygen and pH sensor, and 96-well plates with either oxygen or pH sensors are available. The PhoxyCube system is controlled with intuitive software that has several features, like color coding or grouping of wells, and shows measurement data in real time in graphic representations.
Advantages
- Non-invasive measurements of O2 & pH through well bottom
- Innovative image-based read out compatible with variable plate geometries
- For use in incubator
- Ideally suited for cell cultivation
- Multitude of parallel respiration measurements
- Ready-to-use disposables
- Visualization of kinetics in real-time
Applications exemple
- Simultaneous O2 & pH Monitoring in Cell Culture
- Dose-Response Study in Pharmacological Research
- Respiration Measurements with PhoxyCube
Specifications
| Specifications | Oxygen | pH | |
|---|---|---|---|
| Sensor type | RPSu4 | HP5R-OIW | |
| Measurment range* | 0 – 100 % a. s. 0 – 20.9 % O2 |
5.5 – 8.0 pH | |
| Precision*,** | ± 0.2 % a.s. at 0 % a.s. ± 1.6 % a.s. at 100 % a.s. |
± 0.01 pH at pH = 7 | |
| Drift* | < 1 % a.s. within one week (sampling interval 10 min) | < 0.25 pH units within one week (sampling interval 10 min) in dual analyte mode
< 0.1 pH units within one week (sampling interval 10 min) in single analyte mode |
|
| Response time (t90) at 37 °C | < 60 sec. | < 60 sec. | |
| Device | PhoxyCube Readout Unit | PhoxyCube Connection Box | Power Adapter |
| Type | PhoxyCube v1 or higher | PhoxyCube connection box v2 or higher | TR18RDM180 |
| Input | 18 V DC, 1 A / 18 W | 18 V DC, 1 A / 18 W | 100 / 240 V AC, 50 – 60 Hz, max. 1 A |
| Operating conditions | + 10 °C to + 45 °C 850 hPa to 1100 hPa < 80 % rel. humidity (non-condensing) |
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| Storage conditions | + 5 °C to + 25 °C | ||
| Weight | approx. 1940 g | ||
| Dimensions | 120mm x 190.4 mm x 105.7 mm (Readout Unit) | ||
| *in physiological solutions at 37 °C **typical precision (average of all measurement positions), precision according to ISO 5725-1 |
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