Description
Test for rapid detection of Legionella spp in water samples, based on the combination of immunomagnetic capture and enzyme immunoassay (CEIA).
It is a simple and quick test for presumptive detection of Legionella spp in tap, natural and industrial water. The test combines magnetic immunocapture and enzyme-linked immunoassay (CEIA) with enzymatic colorimetric reaction for quick 1 hour test, after pre-concentrate a sample.
The original sample of water is concentrated by filtration or the like, and this prepared sample is eluted and dispensed into a cuvette to be analyzed by the method CEIA. A suspension of magnetic particles that bind to Legionella is added. If Legionella cells are present in the prepared sample, they will bind to the antibodies immobilized on magnetic particles to form complex bacteria / particle. As these complexes may be separated by a magnet, they are easily washed and resuspended. The complexes are incubated with an anti-Legionella antibody conjugated with an enzyme, to form labeled complexes. After washing the Legionella / particle complexes are visualized colorimetrically, when the enzyme substrates are added.
The limit of detection is about 100 CFU/volume examined, and lower if used with a spectrophotometer.
| Réf. | Tests |
| LGP–10 | PACK 10 TESTS, contains single dose reagents L0/L1/L2/L3/L4/L5, 5 x disposable pipettes, 10 x 3 ml measuring bottles, 10 x 9 ml handling bottles
number of analyzes that can be carried out: 5 to 9 samples (1 control necessary for each series of analyses) |
| LGP–20 | PACK 20 TESTS, contains single dose reagents L0/L1/L2/L3/L4/L5, 20 3 ml measuring bottles, 20 x 9 ml handling bottlesnumber of analyzes that can be carried out: 10 to 19 samples (1 control necessary for each series of analyses) |
| LGP–40 | PACK 40 TESTS, contains single dose reagents L0/L1/L2/L3/L4/L5, 20 3 ml measuring bottles, 40 x 9 ml handling bottlesnumber of analyzes that can be carried out: 20 to 39 samples (1 control necessary for each series of analyses) |
| LGP–100 | PACK 100 TESTS, contains single dose reagents L0/L1/L2/L3/L4/L5, 20 3 ml measuring bottles, 100 x 9 ml handling bottlesnumber of analyzes that can be carried out: 50 to 99 samples (1 control necessary for each series of analyses) |
Important features
Each round of analysis needs its negative control.
It is necessary to filter the samples before analyzing. If you do not have a filter ramp, we have a portable one.
To perform the test you need a special system called MP4 that allows you to make 1 negative control and 3 samples.
To make larger batches, you must purchase a shaker with the necessary racks, also sold by us.
The reading can be done in a laboratory spectrophotometer or in the Primelab 2.0 with the necessary activation ID.
