Description
The test kit offers a rapid, novel, sequential measurement of free-glucose and lactose in low-lactose and lactose-free dairy products. This sequential assay reduces the manual input required by an analyst when compared to traditional lactose assay and therefore improves both accuracy and efficiency. When used in combination with the Megazyme Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products. This is a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase. Lastly, the sensitivity of the assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.
Advantages
- Sequential/High Sensitivity
- Lactose Assay Kit – Sequential/High Sensitivity, 65 assays per kit
- World’s first sequential assay for lactose, i.e. improves accuracy and efficiency
- Contains a specific β-galactosidase for the selective measurement of lactose in dairy products
- Efficient pre-treatment step allows for accurate measurement of lactose in “low-lactose” and “lactose-free” dairy products
- Lower limit of detection (LOD) than any other commercially available enzymatic lactose detection method. LOD at 1.62 mg/L
- Very competitive price (cost per test)
Ref. K-LOLAC
