Description
Infection with the bacterium Listeria monocytogenes leads to a severe clinical picture in infants, immunocompromised individuals and poses significant risks to foetuses, while pregnant women usually have mild symptoms.
This test needs an incubation step of the bacteria on a specific nedieum. The detection is based on a color change with a specific reagent. It can be used for different foods like milk, dairy products, fish.
All kit components are stable until the expiration date on the label. Protect them from light and contamination during use. Do not use the reagents after the expiration date. Store reagents at 2-8°C. Shelf life 12 months from date of production.
Sensitivity: 100% for Listeria monocytogenes
Specificity: 100% for Listeria monocytogenes
Procedure
- Incubation of the sample in suitable nutrient medium (e.g. Ottaviani Agosti Agar Base) for 24 hours at 37⁰C in an incubator
- Open the vial and add one drop or 50 ul of deionised water to the vial.
- Collect a formed colony from the nutrient medium with a swab and dip it into the vial, shaking gently until the contents of the swab are dissolved in the vial.
- Incubate the vial in the incubator at 37⁰C for 6 hours
- Remove the vial from the incubator and check the colour that has appeared: green, positive sample for Listeria monocytogenes
Packaging
- FX100025: 25 vials reagent R
- FX100100; 100 vials reagent R
