Detection of okadaic acid and derivate in bivalve mollusc, OKATEST

Detection of okadaic acid and derivates in bivalve molluscs

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Description

Human consumption of shellfish contaminated with OA-toxins group (OA, DTX1, DTX2 and its esters) causes Diarrheic Shellfish Poisoning (DSP). Inhibition of serine/threonine protein phosphatases (PP1 & PP2A) activity by this group of toxins is assumed to be responsible for these diarrheic disorders.This test is a ready-to-use colorimetric phosphatase inhibition assay for quantification of OA-toxins. OkaTest is performed in a 96-well microtiter plate and is applicable to shellfish such as mussels, clams, oysters and scallops.

OkaTest complies with the legislation requirements for quantitative determination of OA-toxins in molluscs. It complies with the criteria stipulated by the European Reference Laboratory on Marine Toxins and Commission Regulation 15/2012 for determination of OA-group toxins in molluscs, according to the European Commission (DG-SANCO)

Application exemple

Dinophysis species produce lipophilic toxins (diarrhetic shellfish poisoning DSP and pectenotoxins PTX) OKATEST is a kit for the quantitative determination of Okadaic Acid (OA) and other carboxylic toxins of the OA group including DTX1, DTX2 and DTX3 (responsible for diarrheic shellfish poisoning (DSP).

Advantages

  • Fast: 43 samples in about 2 hours
  • Simple: two easy steps after extraction, ready-to-use OA standards
  • Quantitative: 63 -352 µg/Kg
  • Suitable for bivalve molluscs
  • Kit presentation: 48 or 96 tests kits
  • Equipment: plate reader at 405 nm