Description
It is a double test to reveal the presence of milk by combining the detection of Beta-lactoglobulin (Beta-LG), as an indicator for whey proteins, and casein for caseinates. This test will help you to assure a correct milk labelling. This strip test is very easy to implement.
Beta-lactoglobulin, casein, Alpha-lactoalbumin and seroalbumin are the major protein of milk.
This duo test detects the main milk allergen indicators. Therefore, it is especially useful when the source of milk is unknown, such as control of suppliers or in industries where different production processes using whole milk, whey or caseinate are carried out. Single tests targeting only one of these groups of proteins are suitable for detection of milk, but can miss out the present of some byproducts. High temperatures or high pressures as well as cleaning procedures with alkaline detergent can alter the protein structure. Therefore, the double detection of Beta-LG and casein is also recommended in those circumstances as the probability of detecting milk increases.
Milk Detection Kit Features
- Limit of detection (LOD) for Beta-lactoglobulin: 0.1 ppm (mg/kg)
- Limit of detection (LOD) for casein: 1 ppm (mg/kg)
- for example LOD for UHT Milk: 0.05 % (0.05 ml/100 mL)
The test can be used to analyse solid sample and also to analyse working surfaces.
The test was verified on cow milk. When the concentration of allergen is too high, prozone effect can occurs. If the sample has a very high concentration of the allergen, a false negative can appear. For PROTEON DUO MILK, prozone effect can occurs at > 8000 ppm of b-lactoglobulin.
How to use the test ?
- for the surfaces, immerse a cotton swab in 0.5 ml of buffer solution, brush the surface to be tested, put the cotton swab back in the solution for 30 minutes then measure with a strip.
- for solid products, dilute them in 10 mL of buffer solution, crush the mixture then measure on the filtrate.
Supplied equipment: buffer solution, filters, 100 μl pipette tips, tubes, cotton swabs, detection strips
