Description
In the presence of peroxide (H2O2), horse radish peroxidase (HRP) can catalyze the colorless tetramethyl benzidine (TMB), then produces a blue product. The peroxide concentration is measured semi-quantitatively by visual comparison of the reaction zone of the test strip with the fields of a color scale.
This test strip can be used in the semi-quantitative detection of peroxide in milk powder, water and other dairy milk. (please consult the technique for more details if you want to test other samples)
Preparation for the peroxide determination
- Whole milk powder: Weight 1 gram (accurate to 0.01g) of milk powder, then add 8 ml of distilled water to dissolve it.
- Raw milk: No treatment is needed.
- Milk samples should be fully liquid without any agglomeration and deposition. Samples containing more than 25 mg/l H2O2 must be diluted with distilled water. In addition, the pH of the aqueous sample must be within the range 3-12. (Adjust the pH with sodium acetate or hydrochloric acid.)
Features of the peroxide test
- H2O2 meaurement range: 0.5 – 2 – 5 – 10 – 25 mg/l
- result in 20 seconds
Content of the peroxide test
- Tube containing 50 test strips
- Manual 1 piece
Reference: T003/50T
