L-carnitine is a quaternary ammonium compound involved in metabolism in most mammals, plants, and some bacteria. In support of energy metabolism, carnitine transports long-chain fatty
\nacids from the cytosol into mitochondria to be oxidized for free energy production, and also participates in removing products of metabolism from cells.
\nThis kit is applicable for testing of L-carnitine in infant and toddler food and milk powder.<\/p>\n
The current L-carnitine Test Kit is based on an enzymatic assay for the quantitative determination of the total L-carnitine in food, animal feed and pharmaceutical products. The kit principle is accordance with ISO standard. L-carnitine is extracted from sample and then diluted. The diluted sample extract and substrate are added into the microtiter plate well which is coated with enzyme. Incubate in the dark at 20-25oC for 30min. The plate measurement is done using an ELISA reader at 412nm or 405nm. The OD value of the sample can be read from the standard curve.<\/p>\n
Microtiter plate, 8 wells per strip, 6 strips, in total 48 wells
\nSterile water 30mL, 1 bottle
\nConcentrated substrate, 1mL\/bottle, 1 bottle
\nL-cartinine Solid Standard, 2 bottles
\nMicroplate cover, 1 piece<\/p>\n
Potassium hydroxide KOH, 4mol\/L (22.4g KOH dissolve with ultrapure water or deionized water, and dilute to 100mL)
\n13% HClO\u2084 Solution (Take 13ml HClO\u2084and dissolve with ultrapure water or deionized water, and dilute to 100mL)
\nUltrapure water or deionized water
\nAseptic bench
\nELISA reader with 412nm or 405nm
\nWater bath 40\u00b0C
\npH meter
\nCentrifuge \uff1e8000 x g
\nPipette 20-200\u03bcL\uff1b100-1000\u03bcL
\nCentrifuge vials with screw cap 15mL and 50mL
\nVials 1.5-2.0 mL
\nSpiral glass pot 500mL
\nVolumetric flask 100 and 1000mL
\nBeaker 100mL
\n20mL sterile syringes with 0.45\u03bcm filter
\nFilter paper<\/p>\n
stability: 12 months when stored at 2-8\u00b0C<\/p>\n
Ref. R6004<\/p>\n","protected":false},"excerpt":{"rendered":"
Quantitative method for measuring L Carnitine.<\/p>\n","protected":false},"featured_media":5098,"template":"","meta":{"_crdt_document":""},"product_brand":[],"product_cat":[187,367],"product_tag":[],"class_list":{"0":"post-29585","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-analysis","7":"product_cat-chemical-compound","9":"first","10":"instock","11":"taxable","12":"shipping-taxable","13":"product-type-simple"},"yoast_head":"\n