Diagnostic kits

Legipid® Legionella Fast Detection

Test for rapid detection of Legionella spp in water samples, based on the combination of immunomagnetic capture and enzyme immunoassay (CEIA).

It is a simple and quick test for presumptive detection of Legionella spp in tap, natural and industrial water. The test combines magnetic immunocapture and enzyme-linked immunoassay (CEIA) with enzymatic colorimetric reaction for quick 1 hour test, after pre-concentrate a sample.

The original sample of water is concentrated by filtration or the like, and this prepared sample is eluted and dispensed into a cuvette to be analyzed by the method CEIA. A suspension of magnetic particles that bind to Legionella is added. If Legionella cells are present in the prepared sample, they will bind to the antibodies immobilized on magnetic particles to form complex bacteria / particle. As these complexes may be separated by a magnet, they are easily washed and resuspended. The complexes are incubated with an anti-Legionella antibody conjugated with an enzyme, to form labeled complexes. After washing the Legionella / particle complexes are visualized colorimetrically, when the enzyme substrates are added.

The limit of detection is about 100 CFU/volume examined, and lower if used with a spectrophotometer.

Triple Quik Test (Salmonella+Proteus+Brucella)

It is a slide agglutination test for the qualitative and semi-quantitative detection of antibodies anti-Salmonella, Brucella and certain Rickettsias in raw milk. The reagents, standardized suspensions of killed and stained bacteria, agglutinate when mixed with samples containing the homologous antibody. The test can be performed in all foods (each food must be processed accordingly before testing).

Molecular Diagnostic Kits

These detection Kits are designed for the detection of pathogens and viruses for research use only and not for use in diagnostic procedures.

All kits were developed and validated to be used with the following PCR instruments:

• Qiagen Rotor-Gene Q
• BioRad CFX96 Touch Real-Time PCR Detection System

There are 3 different formats available for each pathogen/virus.

1. TaqMan PCR Kit.

• PCR control to monitor for PCR inhibition and validate the quality
• Master Mix for the target and PCR control detection
• Primer and Probe mix
• Positive control and a negative control to confirm the integrity of the kit reagents

2. SYBR Green PCR Kit

• Master Mix for the target detection
• Control Master Mix for the PCR validation
• Primers for the specific amplification of a region of the pathogen genome
• Positive control and a negative control to confirm the integrity of the kit reagents

3. End-Point PCR Kit

• Master Mix and primers for the specific amplification of a region of the pathogen genome
• Positive control and a negative control to confirm the integrity of the kit reagents
• Loading dye and a DNA ladder to facilitate analysis of the results