Legipid® Legionella Fast Detection
Test for rapid detection of Legionella spp in water samples, based on the combination of immunomagnetic capture and enzyme immunoassay (CEIA).
It is a simple and quick test for presumptive detection of Legionella spp in tap, natural and industrial water. The test combines magnetic immunocapture and enzyme-linked immunoassay (CEIA) with enzymatic colorimetric reaction for quick 1 hour test, after pre-concentrate a sample.
The original sample of water is concentrated by filtration or the like, and this prepared sample is eluted and dispensed into a cuvette to be analyzed by the method CEIA. A suspension of magnetic particles that bind to Legionella is added. If Legionella cells are present in the prepared sample, they will bind to the antibodies immobilized on magnetic particles to form complex bacteria / particle. As these complexes may be separated by a magnet, they are easily washed and resuspended. The complexes are incubated with an anti-Legionella antibody conjugated with an enzyme, to form labeled complexes. After washing the Legionella / particle complexes are visualized colorimetrically, when the enzyme substrates are added.
The limit of detection is about 100 CFU/volume examined, and lower if used with a spectrophotometer.
||PACK 10 TESTS
||PACK 40 TESTS
||PACK 100 TESTS